CHS2, a chitin synthase gene from the oomycete Saprolegnia monoica.
Identifieur interne : 002B00 ( Main/Exploration ); précédent : 002A99; suivant : 002B01CHS2, a chitin synthase gene from the oomycete Saprolegnia monoica.
Auteurs : M. Mort-Bontemps [France] ; L. Gay ; M. FèvreSource :
- Microbiology (Reading, England) [ 1350-0872 ] ; 1997.
Descripteurs français
- KwdFr :
- Analyse de séquence d'ADN (MeSH), Chitine synthase (analyse), Chitine synthase (composition chimique), Chitine synthase (génétique), Données de séquences moléculaires (MeSH), Expression des gènes (génétique), Gènes fongiques (génétique), Immunochimie (MeSH), Oomycetes (classification), Oomycetes (composition chimique), Oomycetes (génétique), Similitude de séquences d'acides aminés (MeSH), Séquence d'acides aminés (MeSH), Séquence nucléotidique (MeSH).
- MESH :
- analyse : Chitine synthase.
- composition chimique : Chitine synthase, Oomycetes.
- génétique : Chitine synthase, Expression des gènes, Gènes fongiques, Oomycetes.
- Analyse de séquence d'ADN, Données de séquences moléculaires, Immunochimie, Similitude de séquences d'acides aminés, Séquence d'acides aminés, Séquence nucléotidique.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Base Sequence (MeSH), Chitin Synthase (analysis), Chitin Synthase (chemistry), Chitin Synthase (genetics), Gene Expression (genetics), Genes, Fungal (genetics), Immunochemistry (MeSH), Molecular Sequence Data (MeSH), Oomycetes (chemistry), Oomycetes (classification), Oomycetes (genetics), Sequence Analysis, DNA (MeSH), Sequence Homology, Amino Acid (MeSH).
- MESH :
- chemical , analysis : Chitin Synthase.
- chemical , chemistry : Chitin Synthase.
- chemical , genetics : Chitin Synthase.
- chemistry : Oomycetes.
- classification : Oomycetes.
- genetics : Gene Expression, Genes, Fungal, Oomycetes.
- Amino Acid Sequence, Base Sequence, Immunochemistry, Molecular Sequence Data, Sequence Analysis, DNA, Sequence Homology, Amino Acid.
Abstract
PCR was used to amplify fragments corresponding to the chitin synthase (CHS) genes from the Oomycetes Saprolegnia monoica, Phytophthora capsici and Achlya ambisexualis, utilizing as primers, oligonucleotides designed from the conserved region of CHS genes of chitinous fungi. Chitin synthase homologues were found in the three cellulosic fungi. The chitin synthase 2 gene (CHS2) from S. monoica was cloned, sequenced and characterized. The amino acid sequence deduced from the CHS2 genomic DNA revealed several domains, corresponding to the catalytic domains and polypeptide signatures, of high identity with CHS genes from chitinous fungi. Existence of a CHS gene family in S. monoica was supported by the identification of two CHS sequences among the PCR products, the localization of CHS homologues on two chromosomes, and the detection of two transcripts in mycelia and protoplasts. Polyclonal anti-chitin synthase antibodies raised against the N-terminal and the neutral fragments of the CHS2 products revealed, respectively, two and four proteins in membrane fractions and a truncated active form in entrapped product. The overall comparison of the structure and organization of CHS genes indicates that in spite of their divergent evolution, Oomycetes and chitinous fungi have evolved with conserved chitin synthase systems.
DOI: 10.1099/00221287-143-6-2009
PubMed: 9202477
Affiliations:
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Mort-Bontemps</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Biologie Cellulaire Fongique, Centre de Génétique Moléculaire et Cellulaire, UMR CNRS 5534, Université Lyon, Villeurbanne, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Biologie Cellulaire Fongique, Centre de Génétique Moléculaire et Cellulaire, UMR CNRS 5534, Université Lyon, Villeurbanne</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Villeurbanne</settlement><settlement type="city" wicri:auto="agglo">Lyon</settlement></placeName></affiliation></author><author><name sortKey="Gay, L" sort="Gay, L" uniqKey="Gay L" first="L" last="Gay">L. Gay</name></author><author><name sortKey="Fevre, M" sort="Fevre, M" uniqKey="Fevre M" first="M" last="Fèvre">M. 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Fèvre</name></author></analytic><series><title level="j">Microbiology (Reading, England)</title><idno type="ISSN">1350-0872</idno><imprint><date when="1997" type="published">1997</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term><term>Base Sequence (MeSH)</term><term>Chitin Synthase (analysis)</term><term>Chitin Synthase (chemistry)</term><term>Chitin Synthase (genetics)</term><term>Gene Expression (genetics)</term><term>Genes, Fungal (genetics)</term><term>Immunochemistry (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Oomycetes (chemistry)</term><term>Oomycetes (classification)</term><term>Oomycetes (genetics)</term><term>Sequence Analysis, DNA (MeSH)</term><term>Sequence Homology, Amino Acid (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de séquence d'ADN (MeSH)</term><term>Chitine synthase (analyse)</term><term>Chitine synthase (composition chimique)</term><term>Chitine synthase (génétique)</term><term>Données de séquences moléculaires (MeSH)</term><term>Expression des gènes (génétique)</term><term>Gènes fongiques (génétique)</term><term>Immunochimie (MeSH)</term><term>Oomycetes (classification)</term><term>Oomycetes (composition chimique)</term><term>Oomycetes (génétique)</term><term>Similitude de séquences d'acides aminés (MeSH)</term><term>Séquence d'acides aminés (MeSH)</term><term>Séquence nucléotidique (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Chitin Synthase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Chitin Synthase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Chitin Synthase</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Chitine synthase</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Oomycetes</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Oomycetes</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Chitine synthase</term><term>Oomycetes</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Gene Expression</term><term>Genes, Fungal</term><term>Oomycetes</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Chitine synthase</term><term>Expression des gènes</term><term>Gènes fongiques</term><term>Oomycetes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Base Sequence</term><term>Immunochemistry</term><term>Molecular Sequence Data</term><term>Sequence Analysis, DNA</term><term>Sequence Homology, Amino Acid</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Données de séquences moléculaires</term><term>Immunochimie</term><term>Similitude de séquences d'acides aminés</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">PCR was used to amplify fragments corresponding to the chitin synthase (CHS) genes from the Oomycetes Saprolegnia monoica, Phytophthora capsici and Achlya ambisexualis, utilizing as primers, oligonucleotides designed from the conserved region of CHS genes of chitinous fungi. Chitin synthase homologues were found in the three cellulosic fungi. The chitin synthase 2 gene (CHS2) from S. monoica was cloned, sequenced and characterized. The amino acid sequence deduced from the CHS2 genomic DNA revealed several domains, corresponding to the catalytic domains and polypeptide signatures, of high identity with CHS genes from chitinous fungi. Existence of a CHS gene family in S. monoica was supported by the identification of two CHS sequences among the PCR products, the localization of CHS homologues on two chromosomes, and the detection of two transcripts in mycelia and protoplasts. Polyclonal anti-chitin synthase antibodies raised against the N-terminal and the neutral fragments of the CHS2 products revealed, respectively, two and four proteins in membrane fractions and a truncated active form in entrapped product. The overall comparison of the structure and organization of CHS genes indicates that in spite of their divergent evolution, Oomycetes and chitinous fungi have evolved with conserved chitin synthase systems.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">9202477</PMID><DateCompleted><Year>1997</Year><Month>08</Month><Day>28</Day></DateCompleted><DateRevised><Year>2020</Year><Month>08</Month><Day>26</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1350-0872</ISSN><JournalIssue CitedMedium="Print"><Volume>143 ( Pt 6)</Volume><PubDate><Year>1997</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Microbiology (Reading, England)</Title><ISOAbbreviation>Microbiology (Reading)</ISOAbbreviation></Journal><ArticleTitle>CHS2, a chitin synthase gene from the oomycete Saprolegnia monoica.</ArticleTitle><Pagination><MedlinePgn>2009-20</MedlinePgn></Pagination><Abstract><AbstractText>PCR was used to amplify fragments corresponding to the chitin synthase (CHS) genes from the Oomycetes Saprolegnia monoica, Phytophthora capsici and Achlya ambisexualis, utilizing as primers, oligonucleotides designed from the conserved region of CHS genes of chitinous fungi. Chitin synthase homologues were found in the three cellulosic fungi. The chitin synthase 2 gene (CHS2) from S. monoica was cloned, sequenced and characterized. The amino acid sequence deduced from the CHS2 genomic DNA revealed several domains, corresponding to the catalytic domains and polypeptide signatures, of high identity with CHS genes from chitinous fungi. Existence of a CHS gene family in S. monoica was supported by the identification of two CHS sequences among the PCR products, the localization of CHS homologues on two chromosomes, and the detection of two transcripts in mycelia and protoplasts. Polyclonal anti-chitin synthase antibodies raised against the N-terminal and the neutral fragments of the CHS2 products revealed, respectively, two and four proteins in membrane fractions and a truncated active form in entrapped product. The overall comparison of the structure and organization of CHS genes indicates that in spite of their divergent evolution, Oomycetes and chitinous fungi have evolved with conserved chitin synthase systems.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Mort-Bontemps</LastName><ForeName>M</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Laboratoire de Biologie Cellulaire Fongique, Centre de Génétique Moléculaire et Cellulaire, UMR CNRS 5534, Université Lyon, Villeurbanne, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gay</LastName><ForeName>L</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Fèvre</LastName><ForeName>M</ForeName><Initials>M</Initials></Author></AuthorList><Language>eng</Language><DataBankList CompleteYN="Y"><DataBank><DataBankName>GENBANK</DataBankName><AccessionNumberList><AccessionNumber>U19946</AccessionNumber></AccessionNumberList></DataBank></DataBankList><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Microbiology (Reading)</MedlineTA><NlmUniqueID>9430468</NlmUniqueID><ISSNLinking>1350-0872</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>EC 2.4.1.16</RegistryNumber><NameOfSubstance UI="D002687">Chitin Synthase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002687" MajorTopicYN="N">Chitin Synthase</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015870" MajorTopicYN="N">Gene Expression</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005800" MajorTopicYN="N">Genes, Fungal</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007120" MajorTopicYN="N">Immunochemistry</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009868" MajorTopicYN="N">Oomycetes</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017422" MajorTopicYN="N">Sequence Analysis, DNA</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017386" MajorTopicYN="N">Sequence Homology, Amino Acid</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1997</Year><Month>6</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1997</Year><Month>6</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1997</Year><Month>6</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">9202477</ArticleId><ArticleId IdType="doi">10.1099/00221287-143-6-2009</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>France</li></country><region><li>Auvergne-Rhône-Alpes</li><li>Rhône-Alpes</li></region><settlement><li>Lyon</li><li>Villeurbanne</li></settlement></list><tree><noCountry><name sortKey="Fevre, M" sort="Fevre, M" uniqKey="Fevre M" first="M" last="Fèvre">M. Fèvre</name><name sortKey="Gay, L" sort="Gay, L" uniqKey="Gay L" first="L" last="Gay">L. Gay</name></noCountry><country name="France"><region name="Auvergne-Rhône-Alpes"><name sortKey="Mort Bontemps, M" sort="Mort Bontemps, M" uniqKey="Mort Bontemps M" first="M" last="Mort-Bontemps">M. Mort-Bontemps</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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